Tretinoin nrf2 reporter gene assay

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August undefined, 2024

WebDec 9, 2024 · 双萤光素酶实验的原理. 将目的基因转录调控原件构建入带有荧光素酶 (Firefly luciferase)的表达载体，构建成报告基因质粒，使这段序列调控luciferase的转录表达。. 然后将报告基因质粒转染细胞，给予其不同的处理后裂解细胞，并加入底物荧光素 (luciferin)，luciferase ... WebJun 21, 2024 · To accomplish the aim, we used a combination of a gene reporter assay in immortalized bovine mammary cells, synthetic NRF2 modulators, and blood serum from …

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WebThe ARE Reporter kit is designed for monitoring the activity of the Nrf2 antioxidant pathway in cultured cells. The kit contains a transfection-ready ARE luciferase reporter vector, … WebMar 24, 2024 · Nrf2 is an essential transcription factor regulating the expression of genes containing an electrophile-responsive element (EpRE) responsible for the induction of a wide range of protective enzymes involved in the synthesis of GSH and protection of cells against electrophilic as well as oxidative stress [15,20].Due to the reactive electrophilic activity of … new holland package deals near me

The Influence of Intracellular Glutathione Levels on the Induction …

WebReporter assays provide a way to easily measure a biological response. There are many reporter genes available such as alkaline phosphatase, β-galactosidase, fluorescent proteins and luciferases. Luciferases are used to create bioluminescent reporter assays that are highly quantitative, sensitive across a broad dynamic range, and easily ... WebAug 11, 2009 · (B) Reporter gene assay was performed as described above using NQO1-ARE-dependent luciferase reporter gene construct in MDA-MB-231 cells. (C) Total cell lysates from one of the reporter gene assays were subjected to immunoblot analysis with anti-HA antibodies for detection of Nrf2. WebJan 13, 2012 · 2.4 Gene Fusions. Reporter genes can be used to track the physical location of a segment of DNA or to monitor gene expression. In particular, reporter genes are often incorporated into gene fusions where they are used to follow the level of expression of the target gene. Many genes have products that are complicated or tedious to assay by ... intex swimming pools south africa

ARE-Nrf2 Luciferase Keratinocyte Activation Test Method (OECD …

报告基因检测 Thermo Fisher Scientific - CN

WebDec 4, 2007 · Using a human mammary MCF7-derived AREc32 reporter cell line, we now report that all-trans retinoic acid (ATRA), and other retinoic acid receptor alpha … WebKeratinoSens TM is a cell-based reporter gene assay that models the second key event in the Adverse Outcome Pathway for Dermal Sensitization (keratinocyte activation). The … intex swimming pool salt waterWebApr 15, 2010 · Abstract. The Nrf2 signaling pathway is an important regulator associated with oxidative stress, xenobiotic metabolism, DNA damage and anti-inflammatory response. A key responsive gene in the Nrf2 regulatory pathway is heme oxygense 1 (HO-1), a cytoprotective enzyme that serves in an antioxidant capacity. An imbalance in antioxidant … new holland pack fs19

"WebAug 18, 2024 · Results show that PG was a more potent inducer of Nrf2-mediated gene expression than EGCG, with a 5% benchmark dose (BMD5) of 0.35 µM as compared to 2.45 µM for EGCG in the reporter gene assay. EGC and GA were unable to induce Nrf2-mediated gene expression up to the highest concentration tested (75 µM). " - Tretinoin nrf2 reporter gene assay

Tretinoin nrf2 reporter gene assay

WebMar 25, 2024 · Nrf2 Transcription Factor Assay Kit (Colorimetric) (ab207223) is a high throughput assay to quantify NF-E2-related factor (Nrf2) activation. This assay combines … WebOverexpression of Nrf2 increased the activity of the nrf2 promoter-luciferase reporter, while expression of mutant Nrf2 protein repressed activity. Thus, Nrf2 appears to autoregulate …

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WebApr 15, 2010 · Abstract. The Nrf2 signaling pathway is an important regulator associated with oxidative stress, xenobiotic metabolism, DNA damage and anti-inflammatory … WebABGENEX develops and commercializes novel reagents for the scientific study of human biology and diseases and for the production of new diagnostic assays and potential therapies for such diseases. Areas of biological interest at IMGENEX India include cancer, apoptosis (programmed cell death), molecular signaling pathways, cellular aging, …

WebThe ARE Reporter kit is designed for monitoring the activity of the Nrf2 antioxidant pathway in cultured cells. The kit contains a transfection-ready ARE luciferase reporter vector, … WebThe variability in the results obtained by the participating laboratories was small with mean intra-laboratory standard deviation of 0.16 and mean inter laboratory standard deviation …

Webcysteine residue, resulting in its dissociation from the transcription factor Nrf2 (nuclear factor-erythroid 2-related factor 2). The dissociated Nrf2 can then activate ARE-dependent … WebNrf2 Reporter cell line/Nrf2 cell line can ... Leeporter™ Luciferase Reporter cell line is a stably transfected MCF7 cell line which expresses Renilla luciferase reporter gene under the transcriptional ... plus 2 µ g/ml of Puromycin (Note: Puromycin can be omitted during the reporter cell assays). It is recommended to quickly thaw the ...

WebThe Assay System INDIGO's Human Nrf2 Reporter Cells include the luciferase reporter gene functionally linked to a promoter containing tandem antioxidant response elements (AREs). Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in Nrf2 activity.

WebMay 7, 2013 · The reporter assay demonstrated that Nrf2 induced the promoter activity of Mrp1 gene after co-transfection of pGL3-wt with Nrf2 expressing plasmid (Figure 4, panel … intex sx2100 krystal clear sand filter pumpWebMar 1, 2024 · It was observed that the regulatory region of the NRF2 gene, ... 23 and ABTS 24 assays are in-vitro systems based ... element–driven reporter gene cell line and its use … new holland pagina oficialWebARE-GFP报告基因是翌圣生物自主研发的用于检测ARE转录活性水平为目的的报告基因。ARE(antioxidant response element) 与Nrf2和Nrf1转录因子结合，调节参与保护细胞免受氧化损伤的相关基因。 ARE-GFP报告基因主要应用于检测细胞Antioxidant Response信号通路、药物研究、基因过表达和RNAi的表型分析等。 new holland pa fireworksWebMay 23, 2024 · As a step towards this direction, we previously developed a transient reporter gene assay for analysis of oxidative stress in D. rerio fibroblast (ZF4) and liver (ZFL) cell lines (Lungu-Mitea et al. 2024), by measuring induction of the nuclear factor erythroid 2-related factor 2 (Nrf2), a major upstream regulator of ROS detoxification and … new holland pa fiddlers picnicWebTotal protein was extracted to be used in ELISA assays for mouse Nrf2 (MBS7612500, MyBioSource), IL-1β (ab197742; Abcam), iNOS (MAB9502, R&D Systems), tumor necrosis … intex sx2800WebThe Cignal Reporter Assays are designed for accurate, sensitive and quantitative assessment of the activation of signal transduction pathways. SABiosciences has developed a series of inducible reporter constructs that encode a reporter gene under the control of a basal promoter element (TATA box) joined to tandem repeats of specific new holland pa b\u0026bWebTransient transfection of HepG2 cells with a reporter plasmid of the upstream region of the human PST gene caused a significant increase in reporter gene activity after gallic acid exposure. Moreover, gallic acid increased the nuclear levels of Nrf2, a transcription factor governing antioxidant response element (ARE). new holland pa grocery stores